CCDB Workflow

This diagram provides an overview of the process involved in submitting a sample to the CCDB.

Sample Submissison

See below for detailed instructions on submitting samples to the CCDB.

CCDB Advances

The Canadian Centre for DNA Barcoding periodically provides methods releases in a series called CCDB Advances. These publications provide an overview of new methods applied to areas of DNA barcoding at the CCDB address a broad range of analytical protocols, from specimen imaging, to laboratory methods, and data management.

CCDB Advances – Methods Release 1:
Glass fiber DNA extraction: A new inexpensive method for high throughput DNA isolation

CCDB Advances – Methods Release 2:
Increased DNA barcode recovery using PlatinumĀ® Taq

CCDB Advances – Methods Release 3:
Rapid high-quality imaging of fishes using a flatbed scanner

CCDB Advances – Methods Release 4:
Pre-made frozen PCR and sequencing plates

CCDB Advances – Methods Release 5:
Standardized tissue sampling kits for high throughput DNA barcoding

CCDB Advances – Methods Release 6:
DNA sampling and storage using FTA CloneSaver cards

CCDB Advances – Methods Release 7:
Application of underwater light traps in multi-taxon surveys

CCDB Advances – Methods Release 8:
An electronic lab book to facilitate high-throughput DNA barcoding

CCDB Advances – Methods Release 9:
Automated sequencing clean up: high throughput dye terminator removal

CCDB Protocols

Researchers at the CCDB have developed many protocols to expedite DNA barcoding. These protocols are useful for any lab, large or small, although recent work has focused on developing methods suitable for high-throughput barcode assembly. Further refinements to both lower the cost and boost productivity are a current priority. Additional work seeks to develop more reliable methods for recovering DNA from ancient samples.